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cd36 overexpression knockdown constructs  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology cd36 overexpression knockdown constructs
    Activin A downregulated <t>CD36</t> expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.
    Cd36 Overexpression Knockdown Constructs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+overexpression+knockdown+constructs/pmc07188558-57-8-16?v=Santa+Cruz+Biotechnology
    Average 90 stars, based on 1 article reviews
    cd36 overexpression knockdown constructs - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines"

    Article Title: Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines

    Journal: Biochemical and biophysical research communications

    doi: 10.1016/j.bbrc.2020.03.061

    Activin A downregulated CD36 expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.
    Figure Legend Snippet: Activin A downregulated CD36 expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.

    Techniques Used: Expressing, Microscopy, Transfection, Labeling

    The total production of activin A, in the co-culture assay, was reduced as a result of co-culturing cancer cell lines with the CD36+ fibroblasts. Conditioned medium was collected on (a) day 3 and (b) day 6 and quantified with the ELISA assay. MCF10A is a non-malignant cell line that was used as a reference signal. (c) Production of activin A in monocultures of CD36− fibroblast cells was increased compared to CD36+ fibroblasts.
    Figure Legend Snippet: The total production of activin A, in the co-culture assay, was reduced as a result of co-culturing cancer cell lines with the CD36+ fibroblasts. Conditioned medium was collected on (a) day 3 and (b) day 6 and quantified with the ELISA assay. MCF10A is a non-malignant cell line that was used as a reference signal. (c) Production of activin A in monocultures of CD36− fibroblast cells was increased compared to CD36+ fibroblasts.

    Techniques Used: Co-culture Assay, Enzyme-linked Immunosorbent Assay

    The co-culture of breast cancer cell lines with CD36+ fibroblasts showed growth inhibition, reversion of basal and lateral polarity, or reduced malignant potential. (a-d) Co-culture of MDA-MB-231 with CD36+ or CD36− fibroblasts resulted in growth inhibition or promotion, respectively; two populations of Ki67 expressing cells; and higher frequency of proliferating cells, with high-level of Ki67, with the CD36− co-culture. (e-g) Co-culture of MCF7 with CD36+ induced renormalization of lateral and basal polarity, reduced colony formation, and reduced number of single cells. (h-i) Preconditioned MDA-MB-231 cells to CD36+ fibroblasts show reduced malignant potential as indicated with the AIG assay, and preconditioned MCF7 cells to CD36+ fibroblasts show no significant statistical difference.
    Figure Legend Snippet: The co-culture of breast cancer cell lines with CD36+ fibroblasts showed growth inhibition, reversion of basal and lateral polarity, or reduced malignant potential. (a-d) Co-culture of MDA-MB-231 with CD36+ or CD36− fibroblasts resulted in growth inhibition or promotion, respectively; two populations of Ki67 expressing cells; and higher frequency of proliferating cells, with high-level of Ki67, with the CD36− co-culture. (e-g) Co-culture of MCF7 with CD36+ induced renormalization of lateral and basal polarity, reduced colony formation, and reduced number of single cells. (h-i) Preconditioned MDA-MB-231 cells to CD36+ fibroblasts show reduced malignant potential as indicated with the AIG assay, and preconditioned MCF7 cells to CD36+ fibroblasts show no significant statistical difference.

    Techniques Used: Co-Culture Assay, Inhibition, Expressing

    YY1 and pSmad 2/3 expressions were downregulated in organoid models of MCF7 and MDA-MB-231, co-cultured with CD36+ fibroblasts, respectively.
    Figure Legend Snippet: YY1 and pSmad 2/3 expressions were downregulated in organoid models of MCF7 and MDA-MB-231, co-cultured with CD36+ fibroblasts, respectively.

    Techniques Used: Cell Culture



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    cd36 overexpression knockdown constructs  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology cd36 overexpression knockdown constructs
    Activin A downregulated <t>CD36</t> expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.
    Cd36 Overexpression Knockdown Constructs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+overexpression+knockdown+constructs/pmc07188558-57-8-16?v=Santa+Cruz+Biotechnology
    Average 90 stars, based on 1 article reviews
    cd36 overexpression knockdown constructs - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Activin A downregulated CD36 expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.

    Journal: Biochemical and biophysical research communications

    Article Title: Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines

    doi: 10.1016/j.bbrc.2020.03.061

    Figure Lengend Snippet: Activin A downregulated CD36 expression in primary fibroblasts as monitored with immunofluorescent wide-field microscopy. (a) CD36 expression in transfected primary fibroblasts was monitored with a wide-field fluorescent microscope, where nuclei were labeled with DAPI and delineated computationally in yellow. (b) CD36 expression of primary fibroblasts, cocultured with MDA-MB-231 cells, was quantitatively analyzed. Controls were primary, CD36+-, CD36−-, primary fibroblasts, and fibroblast with MDA-MB-231 coculture, supplemented with activin A neutralizing antibody. (c) CD36 expression of primary fibroblasts, in response to different concentrations of activin A at 0, 1, 2.25, and 20 ng/mL and the conditioned media from MDA-MB-231 conditioned medium were quantified. Controls were CD36+-, CD36−-, primary fibroblasts and the conditioned media supplemented with the activin A neutralizing antibody.

    Article Snippet: CD36 transfection and validation Fibroblasts were transfected with CD36 overexpression and knockdown constructs using CRISPR-cas9 technology (Santa Cruz Biotechnology SC400233-ACT/KO) and validated using fluorescent microscopy per vendor’s protocol.

    Techniques: Expressing, Microscopy, Transfection, Labeling

    The total production of activin A, in the co-culture assay, was reduced as a result of co-culturing cancer cell lines with the CD36+ fibroblasts. Conditioned medium was collected on (a) day 3 and (b) day 6 and quantified with the ELISA assay. MCF10A is a non-malignant cell line that was used as a reference signal. (c) Production of activin A in monocultures of CD36− fibroblast cells was increased compared to CD36+ fibroblasts.

    Journal: Biochemical and biophysical research communications

    Article Title: Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines

    doi: 10.1016/j.bbrc.2020.03.061

    Figure Lengend Snippet: The total production of activin A, in the co-culture assay, was reduced as a result of co-culturing cancer cell lines with the CD36+ fibroblasts. Conditioned medium was collected on (a) day 3 and (b) day 6 and quantified with the ELISA assay. MCF10A is a non-malignant cell line that was used as a reference signal. (c) Production of activin A in monocultures of CD36− fibroblast cells was increased compared to CD36+ fibroblasts.

    Article Snippet: CD36 transfection and validation Fibroblasts were transfected with CD36 overexpression and knockdown constructs using CRISPR-cas9 technology (Santa Cruz Biotechnology SC400233-ACT/KO) and validated using fluorescent microscopy per vendor’s protocol.

    Techniques: Co-culture Assay, Enzyme-linked Immunosorbent Assay

    The co-culture of breast cancer cell lines with CD36+ fibroblasts showed growth inhibition, reversion of basal and lateral polarity, or reduced malignant potential. (a-d) Co-culture of MDA-MB-231 with CD36+ or CD36− fibroblasts resulted in growth inhibition or promotion, respectively; two populations of Ki67 expressing cells; and higher frequency of proliferating cells, with high-level of Ki67, with the CD36− co-culture. (e-g) Co-culture of MCF7 with CD36+ induced renormalization of lateral and basal polarity, reduced colony formation, and reduced number of single cells. (h-i) Preconditioned MDA-MB-231 cells to CD36+ fibroblasts show reduced malignant potential as indicated with the AIG assay, and preconditioned MCF7 cells to CD36+ fibroblasts show no significant statistical difference.

    Journal: Biochemical and biophysical research communications

    Article Title: Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines

    doi: 10.1016/j.bbrc.2020.03.061

    Figure Lengend Snippet: The co-culture of breast cancer cell lines with CD36+ fibroblasts showed growth inhibition, reversion of basal and lateral polarity, or reduced malignant potential. (a-d) Co-culture of MDA-MB-231 with CD36+ or CD36− fibroblasts resulted in growth inhibition or promotion, respectively; two populations of Ki67 expressing cells; and higher frequency of proliferating cells, with high-level of Ki67, with the CD36− co-culture. (e-g) Co-culture of MCF7 with CD36+ induced renormalization of lateral and basal polarity, reduced colony formation, and reduced number of single cells. (h-i) Preconditioned MDA-MB-231 cells to CD36+ fibroblasts show reduced malignant potential as indicated with the AIG assay, and preconditioned MCF7 cells to CD36+ fibroblasts show no significant statistical difference.

    Article Snippet: CD36 transfection and validation Fibroblasts were transfected with CD36 overexpression and knockdown constructs using CRISPR-cas9 technology (Santa Cruz Biotechnology SC400233-ACT/KO) and validated using fluorescent microscopy per vendor’s protocol.

    Techniques: Co-Culture Assay, Inhibition, Expressing

    YY1 and pSmad 2/3 expressions were downregulated in organoid models of MCF7 and MDA-MB-231, co-cultured with CD36+ fibroblasts, respectively.

    Journal: Biochemical and biophysical research communications

    Article Title: Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines

    doi: 10.1016/j.bbrc.2020.03.061

    Figure Lengend Snippet: YY1 and pSmad 2/3 expressions were downregulated in organoid models of MCF7 and MDA-MB-231, co-cultured with CD36+ fibroblasts, respectively.

    Article Snippet: CD36 transfection and validation Fibroblasts were transfected with CD36 overexpression and knockdown constructs using CRISPR-cas9 technology (Santa Cruz Biotechnology SC400233-ACT/KO) and validated using fluorescent microscopy per vendor’s protocol.

    Techniques: Cell Culture